Issue 40, 2020
From the journal:

Chemical Communications

A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity†

Yanglingzhi Chen,a   Keiji Murayama,a   Hiromu Kashida, ORCID logo a   Yukiko Kamiya ORCID logo a  and  Hiroyuki Asanuma ORCID logo *a  

* Corresponding authors

a Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
E-mail: asanuma@chembio.nagoya-u.ac.jp

Abstract

A triplex-forming oligonucleotide (TFO) linear probe containing perylene derivatives was synthesized. The TFO linear probe formed a remarkably stable triplex with a target DNA duplex, resulting in the light-up of fluorescence emission. The sensitivity was extremely high even at pH 7. Detection of PCR-amplified target DNA was demonstrated.

Graphical abstract: A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity

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Article information

DOI
https://doi.org/10.1039/D0CC01865A
Article type
Communication
Submitted
11 Mar 2020
Accepted
17 Apr 2020
First published
17 Apr 2020

Chem. Commun., 2020,56, 5358-5361

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A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity

Y. Chen, K. Murayama, H. Kashida, Y. Kamiya and H. Asanuma, Chem. Commun., 2020, 56, 5358 DOI: 10.1039/D0CC01865A

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