Issue 49, 2020
From the journal:

Chemical Communications

Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA)†

Stephan Sinn, ORCID logo *a   Joana Krämer ORCID logo a  and  Frank Biedermann ORCID logo *a  

* Corresponding authors

a Karlsruhe Institute of Technology (KIT), Institute of Nanotechnology (INT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
E-mail: stephan.sinn@kit.edu, frank.biedermann@kit.edu

Abstract

A simple change has important consequences: the guest-displacement assay (GDA) is introduced which allows for binding affinity determinations of supramolecular complexes with spectroscopically silent hosts and guests. GDA is complementary to indicator-displacement assay for affinity measurements with soluble components, but is superior for insoluble or for weakly binding guests.

Graphical abstract: Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA)

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Article information

DOI
https://doi.org/10.1039/D0CC01841D
Article type
Communication
Submitted
10 Mar 2020
Accepted
28 Apr 2020
First published
01 May 2020
This article is Open Access
Creative Commons BY license

Chem. Commun., 2020,56, 6620-6623

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Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA)

S. Sinn, J. Krämer and F. Biedermann, Chem. Commun., 2020, 56, 6620 DOI: 10.1039/D0CC01841D

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