Issue 39, 2020

Label-free and amplified detection of apoptosis-associated caspase activity using branched rolling circle amplification

Abstract

We develop a label-free fluorescence method for ultrasensitive detection of apoptosis-associated caspase activity based on branched rolling circle amplification. This work demonstrates for the first time the use of a nucleic acid amplification strategy for the amplified detection of caspase activity, which allows for highly sensitive detection of endogenous caspase even at the single-cell level. This method can be further applied for kinetic analysis and inhibitor screening.

Graphical abstract: Label-free and amplified detection of apoptosis-associated caspase activity using branched rolling circle amplification

Supplementary files

Article information

Article type
Communication
Submitted
28 Feb 2020
Accepted
30 Mar 2020
First published
31 Mar 2020

Chem. Commun., 2020,56, 5243-5246

Label-free and amplified detection of apoptosis-associated caspase activity using branched rolling circle amplification

M. Liu, D. Zhang, X. Zhang, Q. Xu, F. Ma and C. Zhang, Chem. Commun., 2020, 56, 5243 DOI: 10.1039/D0CC01564D

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