Stabilizing synthetic DNA for long-term data storage with earth alkaline salts

A. Xavier Kohll; Philipp L. Antkowiak; Weida D. Chen; Bichlien H. Nguyen; Wendelin J. Stark; Luis Ceze; Karin Strauss; Robert N. Grass

doi:10.1039/D0CC00222D

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Stabilizing synthetic DNA for long-term data storage with earth alkaline salts†

A. Xavier Kohll,^a Philipp L. Antkowiak,

^a Weida D. Chen,^a Bichlien H. Nguyen,^b Wendelin J. Stark,

^a Luis Ceze,

^c Karin Strauss

^b and Robert N. Grass

*^a

Author affiliations

* Corresponding authors

^a Institute for Chemical and Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland
E-mail: rograss@ethz.ch

^b Microsoft, One Microsoft Way, Redmond, WA, USA

^c Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA

Abstract

Rapid aging tests (70 °C, 50% RH) of solid state DNA dried in the presence of various salt formulations, showed the strong stabilizing effect of calcium phosphate, calcium chloride and magnesium chloride, even at high DNA loadings (>20 wt%). A DNA-based digital information storage system utilizing the stabilizing effect of MgCl₂ was tested by storing a DNA file, encoding 115 kB of digital data, and the successful readout of the file by sequencing after accelerated aging.

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DOI: https://doi.org/10.1039/D0CC00222D
Article type: Communication
Submitted: 09 Jan 2020
Accepted: 19 Feb 2020
First published: 24 Feb 2020
This article is Open Access

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Chem. Commun., 2020,56, 3613-3616

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Stabilizing synthetic DNA for long-term data storage with earth alkaline salts

A. X. Kohll, P. L. Antkowiak, W. D. Chen, B. H. Nguyen, W. J. Stark, L. Ceze, K. Strauss and R. N. Grass, Chem. Commun., 2020, 56, 3613 DOI: 10.1039/D0CC00222D

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Chemical Communications

Stabilizing synthetic DNA for long-term data storage with earth alkaline salts†

Abstract

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Stabilizing synthetic DNA for long-term data storage with earth alkaline salts

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