Issue 30, 2020

Improving resolving ability of expansion microscopy by varying crosslinker concentration

Abstract

Here, we systematically investigated the performance of expansion microscopy (ExM) with different crosslinker concentrations. We modified ExM with 0.06% N,N′-methylenebisacrylamide (MBAA) (termed 0.06%-MBAA ExM), increased the expansion factor to 5.7 and achieved a lateral resolution of ∼50 nm with a common confocal microscope.

Graphical abstract: Improving resolving ability of expansion microscopy by varying crosslinker concentration

Supplementary files

Article information

Article type
Communication
Submitted
03 Jan 2020
Accepted
05 Mar 2020
First published
06 Mar 2020

Chem. Commun., 2020,56, 4176-4179

Improving resolving ability of expansion microscopy by varying crosslinker concentration

Y. Hu, X. Chu, T. Chen, Q. Pan, C. Liu, J. Yi and X. Chu, Chem. Commun., 2020, 56, 4176 DOI: 10.1039/D0CC00052C

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