Host–guest recognition coupled with triple signal amplification endows an electrochemiluminescent biosensor with enhanced sensitivity

Abstract

We demonstrate for the first time that host–guest recognition coupled with triple signal amplification endows an electrochemiluminescent (ECL) biosensor with enhanced sensitivity for uracil DNA glycosylase (UDG) assay. This biosensor exhibits good selectivity and extremely high sensitivity, and it can be used to screen UDG inhibitors and measure the cellular UDG activity as well.