Sensitive and specific detection of tumour cells based on a multivalent DNA nanocreeper and a multiplexed fluorescence supersandwich

Lei Luo; Wenshan Li; Dongliu Xiang; Yanyun Ma; Yuan Zhou; Yaqing Xu; Nandi Chen; Qing Wang; Jin Huang; Jianbo Liu; Xiaohai Yang; Kemin Wang

doi:10.1039/C9CC08618H

Sensitive and specific detection of tumour cells based on a multivalent DNA nanocreeper and a multiplexed fluorescence supersandwich†

Lei Luo,^a Wenshan Li,

^b Dongliu Xiang,^a Yanyun Ma,^a Yuan Zhou,^a Yaqing Xu,^a Nandi Chen,^c Qing Wang,^a Jin Huang,

^a Jianbo Liu,

^a Xiaohai Yang

*^a and Kemin Wang

*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China
E-mail: yangxiaohai@hnu.edu.cn, kmwang@hnu.edu.cn
Fax: +86-731-88821566
Tel: +86-731-88821566

^b College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China

^c Department of Gastrointestinal Surgery, The Second Clinical Medicine College (Shenzhen People's Hospital) of Jinan University, Shenzhen, Guangdong 518020, China

Abstract

A self-assembled DNA nanostructure based on a DNA nanocreeper and multiplexed fluorescence supersandwich was designed for the sensitive and specific detection of tumour cells. This nanostructure could improve the binding affinity of current aptamers and trigger signal amplification, which provide potential for the discrimination of low abundant target cells in liquid biopsy.

Chemical Communications

Sensitive and specific detection of tumour cells based on a multivalent DNA nanocreeper and a multiplexed fluorescence supersandwich†

Abstract

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Sensitive and specific detection of tumour cells based on a multivalent DNA nanocreeper and a multiplexed fluorescence supersandwich

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