Facile fabrication of a controlled polymer brush-type functional nanoprobe for highly sensitive determination of alpha fetoprotein

Abstract

As robust functional polymers, polymer brush-based hybrid nanomaterials have potential application in the highly sensitive determination of tumor markers (TMs). Currently, there are plentiful reports on the polymerization methods of functional polymer brushes. Low ppm ATRP (activators (re)generated by electron transfer for atom transfer radical polymerization (A(R)GET ATRP), initiators for continuous activator regeneration atom transfer radical polymerization (ICAR ATRP) and electrochemically mediated atom transfer radical polymerization (eATRP)) is a facile and robust methodology with the advantages of simplicity, eco-friendliness and wide applicability to prepare well-defined polymeric materials. In this work, a controlled polymer brush-type functional nanoprobe is successfully fabricated by functional AGET ATRP and used as a sandwich-type electrochemical immunosensor for precise detection of TMs (alpha-fetoprotein, AFP). Using graphene oxide (GO) as an excellent conductive matrix, a GO-based poly-heterozygosis pyridine nanomaterial (GO@PHPY) is obtained by surface-initiated AGET ATRP and photocatalytic modification. The nanoprobe is assembled using GO@PHPY and a detection antibody (Ab2) to detect AFP, in which Cu(II) serves as a signal label to coordinate with the pyridyl group. Under optimized conditions, the electrochemical sensor exhibits a good detection effect on AFP, with a detection range of 0.1 pg mL⁻¹ to 100 ng mL⁻¹ and a low detection limit of 0.08 pg mL⁻¹. It is worth noting that the detection platform can be applied to the detection of real human serum samples. Thus, it is a desirable platform for AFP detection in clinical diagnosis and practical applications. Meanwhile, this work proves that designing and constructing functional polymer brushes is one of the most effective methods for developing new materials for analytical scientific applications.