Issue 46, 2020

A lateral flow biosensor based on gold nanoparticles detects four hemorrhagic fever viruses

Abstract

The pathogen of viral hemorrhagic fever (VHF), which is harmful to human health, is a hemorrhagic fever virus. Clinicians have long needed convenient and sensitive point-of-care rapid diagnostic tests (RDTs) for hemorrhagic fever viruses. Commonly used methods for pathogen detection rely on conventional culture-based tests, antibody-based assays and polymerase chain reaction (PCR)-based techniques. However, these methods are costly, laborious and time-consuming. Herein, we present a simple and sensitive biosensor for the rapid detection of hemorrhagic fever viruses. For this assay, we develop lateral flow biosensors (LFBs) based on magnetic beads and nicking enzyme-assisted isothermal strand-displacement amplification (SDA) for the detection of hemorrhagic fever viruses. The detection limit of this assay is 10 fM.

Graphical abstract: A lateral flow biosensor based on gold nanoparticles detects four hemorrhagic fever viruses

Article information

Article type
Paper
Submitted
07 Jun 2020
Accepted
05 Oct 2020
First published
07 Oct 2020

Anal. Methods, 2020,12, 5613-5620

A lateral flow biosensor based on gold nanoparticles detects four hemorrhagic fever viruses

J. Zhao, S. Fang, Y. Liu, L. Zeng and Z. He, Anal. Methods, 2020, 12, 5613 DOI: 10.1039/D0AY01137A

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