Issue 30, 2020

Disposable biogenic amine biosensors for histamine determination in fish

Abstract

This study presents the development of disposable biosensors employed in the determination of histamine in fish samples. Screen printed carbon electrodes (SPCEs) were first modified with a mixture of titanium dioxide nanoparticles (TiO2), carboxylated multiwalled carbon nanotubes (c-MWCNTs), hexaammineruthenium(III) chloride (RU) and chitosan (CS). Diamine oxidase (DAO) or monoamine oxidase (MAO) enzymes were further immobilized onto the TiO2–c-MWCNT–RU–CS/SPCEs via 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC) and hydroxysuccinimide (NHS) chemistry for the fabrication of the biosensors. The morphological and electrochemical properties of the proposed biosensors were studied using scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, cyclic voltammetry (CV), chronoamperometry and electrochemical impedance spectroscopy (EIS). A performance comparison of two biosensors indicated that the one based on DAO had a linear concentration range from 9.9 × 10−6 to 1.1 × 10−3 M and the other based on MAO, from 5.6 × 10−5 to 1.1 × 10−3 M for histamine. The sensitivity of the DAO based biosensor was almost 1.5 times higher than that of the MAO based biosensor. The proposed biosensors were successfully employed to determine histamine in fish samples and the recoveries were between 100.0% and 104.6%.

Graphical abstract: Disposable biogenic amine biosensors for histamine determination in fish

Supplementary files

Article information

Article type
Paper
Submitted
19 Apr 2020
Accepted
21 Jun 2020
First published
24 Jun 2020

Anal. Methods, 2020,12, 3802-3812

Disposable biogenic amine biosensors for histamine determination in fish

İ. O. Koçoğlu, P. E. Erden and E. Kılıç, Anal. Methods, 2020, 12, 3802 DOI: 10.1039/D0AY00802H

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