Issue 18, 2020

Ultrasensitive determination of ractopamine based on dual catalytic signal amplification by Pd nanocubes and HRP using a flow injection chemiluminescence immunoassay

Abstract

A rapid and highly sensitive flow injection chemiluminescence immunoassay was developed to detect ractopamine residues in pork products. Palladium nanocubes with excellent catalytic performance for a traditional luminol–PIP–H2O2 chemiluminescence system were used as effective carriers to connect a ractopamine antibody and horseradish peroxidase (HRP). Double amplification of a chemiluminescence signal was realized because of palladium nanocubes and HRP. Carboxyl-modified resin beads were used as suitable materials to load ractopamine-coating antigens due to their good biocompatibility and large specific surface area. Based on the principle of competitive immunity, ractopamine standard solution would compete with antigen loaded on carboxyl resin beads for limited binding sites on a ractopamine antibody. Thus, the chemiluminescence intensity of an immunosensor has a linear negative correlation with the logarithm value of ractopamine concentration. Under optimal experimental conditions, the detection range of ractopamine was 0.005–1000 ng mL−1, and the limit of detection (LOD) was 1.7 pg mL−1 (S/N = 3). The proposed immunoassay possessed acceptable accuracy, high specificity and reproducibility and RAC was examined in pork and pig feed with satisfactory results, which would provide a better prospect for the detection of small molecules in food and environment analysis.

Graphical abstract: Ultrasensitive determination of ractopamine based on dual catalytic signal amplification by Pd nanocubes and HRP using a flow injection chemiluminescence immunoassay

Article information

Article type
Paper
Submitted
16 Jun 2020
Accepted
10 Jul 2020
First published
10 Jul 2020

Analyst, 2020,145, 6171-6179

Ultrasensitive determination of ractopamine based on dual catalytic signal amplification by Pd nanocubes and HRP using a flow injection chemiluminescence immunoassay

Y. Pei, J. Zhang, K. Wu, A. Deng and J. Li, Analyst, 2020, 145, 6171 DOI: 10.1039/D0AN01203C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements