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Sensitive colorimetric sensor for point-of-care detection of acetylcholinesterase using cobalt oxyhydroxide nanoflakes

Abstract

Point-of-care monitoring of acetylcholinesterase (AChE) is of significant importance for pesticide poisoning and disease diagnosis due to it plays a pivotal role in biological nerve conduction system. Herein, we designed a colorimetric strategy for the facile and accurate detection of AChE based on tandem catalysis with multi-enzyme system, which constituted by cobalt oxyhydroxide nanoflakes (CoOOH NFs) and choline oxidase (CHO). In this sensor, AChE catalytically hydrolyzed acetylcholine (ACh) to produce choline, further efficiently oxidized by CHO to yield H2O2. CoOOH NFs as a nanozyme could efficiently catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxTMB with the help of H2O2, accompanying the enhancement of absorbance intensity. The resulting intensity could be employed as the signal output of CHO/CoOOH/ACh system for monitoring AChE. Under optimal conditions, the developed sensor possessed sensitive responsed to AChE with a detection limit of 33 μU mL–1. Interestingly, the proposed platform was applied to fabricate paper-based sensor for rapidly recognizing AChE by direct observation with the naked eyes. Combining with smartphone and ImageJ software, we further developed an image processing algorithm for quantitative detection of AChE with highly promising, which validated the outstanding potential on-site application in clinical diagnostics and pesticide poisoning.

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Publication details

The article was received on 13 Nov 2018, accepted on 12 Jan 2019 and first published on 14 Jan 2019


Article type: Paper
DOI: 10.1039/C8TB02987C
Citation: J. Mater. Chem. B, 2019, Accepted Manuscript
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    Sensitive colorimetric sensor for point-of-care detection of acetylcholinesterase using cobalt oxyhydroxide nanoflakes

    R. Jin, Z. Xing, D. Kong, X. Yan, F. Liu, Y. Gao, P. sun, X. Liang and G. Lu, J. Mater. Chem. B, 2019, Accepted Manuscript , DOI: 10.1039/C8TB02987C

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