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Photo-cleavable purification/protection handle assisted synthesis of giant modified proteins with tandem repeats

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Abstract

Proteins with tandem repeats have essential physical or biological roles in cells and have been widely investigated as biomaterials or vaccines. Chemically derived proteins with tandem repeats would be beneficial for research, owing to their accurate structures, possibly with precise modifications, produced by chemical synthesis. Traditional protein synthesis often leads to severe handling loss due to multiple ligations and HPLC purifications. To improve the protein synthesis efficiency, we developed a purification/protection handle consisting of a His6 tag and a photo-labile linker. This handle has great potential to facilitate purification with immobilized metal affinity chromatography techniques and also provides orthogonal protection for N-terminal Cys. The synthesis of the model proteins Muc1 and antifreeze glycoprotein mimics shows that the handle decreases the requirement for HPLC and enables both convergent and sequential assembly of peptide segments.

Graphical abstract: Photo-cleavable purification/protection handle assisted synthesis of giant modified proteins with tandem repeats

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Publication details

The article was received on 26 Jul 2019, accepted on 04 Aug 2019 and first published on 12 Aug 2019


Article type: Edge Article
DOI: 10.1039/C9SC03693H
Chem. Sci., 2019, Advance Article
  • Open access: Creative Commons BY license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Photo-cleavable purification/protection handle assisted synthesis of giant modified proteins with tandem repeats

    X. Liu, J. Liu, Z. Wu, L. Chen, S. Wang and P. Wang, Chem. Sci., 2019, Advance Article , DOI: 10.1039/C9SC03693H

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