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Issue 22, 2019
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Continuous nonenzymatic cross-replication of DNA strands with in situ activated DNA oligonucleotides

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Abstract

Continuous enzyme-free replication of oligonucleotides is central for open-ended evolution experiments that mimic the origin of life. Here, we studied a reaction system, whereby two 24mer DNA templates cross-catalyzed each other's synthesis from four 12mer DNA fragments, two of which were in situ activated with the condensing agent 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC). We circumvented the problem of product inhibition by melting the stable product duplexes for their reuse as templates in the following ligation step. The system reproduced itself through ligation/melting cycles and survived exponential dilution. We quantified EDC-induced side reactions in a detailed kinetic model. The model allowed us to analyze the effects of various reaction rates on the system's kinetics and confirmed maximal replication under the chosen conditions. The presented system enables us to study nonenzymatic open-ended evolution experiments starting from diverse sequence pools.

Graphical abstract: Continuous nonenzymatic cross-replication of DNA strands with in situ activated DNA oligonucleotides

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Publication details

The article was received on 14 Feb 2019, accepted on 28 Apr 2019 and first published on 30 Apr 2019


Article type: Edge Article
DOI: 10.1039/C9SC00770A
Chem. Sci., 2019,10, 5807-5814
  • Open access: Creative Commons BY-NC license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Continuous nonenzymatic cross-replication of DNA strands with in situ activated DNA oligonucleotides

    E. Edeleva, A. Salditt, J. Stamp, P. Schwintek, J. Boekhoven and D. Braun, Chem. Sci., 2019, 10, 5807
    DOI: 10.1039/C9SC00770A

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