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Issue 8, 2019
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Lysosome-targeted photoactivation reveals local sphingosine metabolism signatures

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Abstract

Lipids are essential components of eukaryotic cell membranes and play crucial roles in cellular signaling and metabolism. While increasing evidence shows that the activities of lipids are dependent upon subcellular localization, tools to study local lipid metabolism and signaling are limited. Herein, we report an approach that enabled us to selectively deliver photo-caged lipids into lysosomes and thereafter to quickly release the lipid molecules by illumination. On combining this method with genetic techniques and lipidomics, we were able to investigate the localization-dependent metabolism of an important intermediate of sphingolipid metabolism, sphingosine. Our data reveal a distinct metabolic pattern of lysosomal sphingosine. In general, this method has the potential to serve as a platform to study lysosomal metabolism and signaling of various lipids and metabolites in living cells.

Graphical abstract: Lysosome-targeted photoactivation reveals local sphingosine metabolism signatures

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Publication details

The article was received on 14 Aug 2018, accepted on 21 Dec 2018 and first published on 26 Dec 2018


Article type: Edge Article
DOI: 10.1039/C8SC03614D
Citation: Chem. Sci., 2019,10, 2253-2258
  • Open access: Creative Commons BY-NC license
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    Lysosome-targeted photoactivation reveals local sphingosine metabolism signatures

    S. Feng, T. Harayama, D. Chang, J. T. Hannich, N. Winssinger and H. Riezman, Chem. Sci., 2019, 10, 2253
    DOI: 10.1039/C8SC03614D

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