Jump to main content
Jump to site search


A non-sacrificial method for the quantification of poly(ethylene glycol) grafting density on gold nanoparticles for applications in nanomedicine

Author affiliations

Abstract

The grafting density of poly(ethylene glycol) (PEG) on nanoparticle (NP) surfaces is the most important parameter determining the interaction of nanoparticles with serum proteins, the subsequent sequestration of the nanoparticle from the bloodstream by the mononuclear phagocyte system, and the eventual delivery efficiency to tumor tissues. However, the majority of in vivo studies do not characterize or report the grafting density of PEG on nanoparticles due to a lack of feasible characterization methods, making it difficult to evaluate the published studies and reconcile apparent conflicting results. Herein, we develop a facile and non-sacrificial 1H NMR analytical approach for the quantitative characterization of grafting density of thiol-terminated PEG (HS-PEG) on gold NPs (GNPs). A multi-Lorentzian-splitting algorithm is used to distinguish the NMR signal of free PEG from those of the grafted ones, therefore allowing in situ monitoring of the grafting process to study the effects of GNP sizes, PEG molecular weights and NP capping ligands on grafting rates and grafting densities. The main advantage of this method is that it is not limited by the types of terminal functional groups on PEG, surface chemistry of the nanoparticles or their composition. It also provides a set of critical and standard guides for characterization of the PEG grafting density on nanoparticles for in vivo biological and biomedical studies.

Graphical abstract: A non-sacrificial method for the quantification of poly(ethylene glycol) grafting density on gold nanoparticles for applications in nanomedicine

Back to tab navigation

Supplementary files

Publication details

The article was received on 28 Jun 2018, accepted on 11 Dec 2018 and first published on 14 Dec 2018


Article type: Edge Article
DOI: 10.1039/C8SC02847H
Citation: Chem. Sci., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    A non-sacrificial method for the quantification of poly(ethylene glycol) grafting density on gold nanoparticles for applications in nanomedicine

    J. Lu, Y. Xue, R. Shi, J. Kang, C. Zhao, N. Zhang, C. Wang, Z. Lu and K. Liu, Chem. Sci., 2019, Advance Article , DOI: 10.1039/C8SC02847H

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements