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Issue 61, 2019
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Bulky cations greatly increase the turnover of a native hammerhead ribozyme

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Abstract

Methods to facilitate the catalytic turnover of ribozymes are required for advancing oligonucleotide-based technologies. This study examined tetraalkylammonium ions for their ability to increase the efficiency of catalytic turnover of a native hammerhead ribozyme. Kinetic analysis showed that large tetraalkylammonium ions significantly increased the turnover rate of the ribozyme and was much more effective than poly(ethylene glycol) (PEG) and urea. The magnitude of the rate increase depended on the concentrations of Mg2+ and tetrapentylammonium ions, and the rate was enhanced by more than 180-fold at the optimal concentrations of these salts. The results provide physical insights into interactions of ribozymes with large cationic molecules through electrostatic forces and steric hindrance.

Graphical abstract: Bulky cations greatly increase the turnover of a native hammerhead ribozyme

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Supplementary files

Article information


Submitted
28 Aug 2019
Accepted
28 Oct 2019
First published
04 Nov 2019

This article is Open Access

RSC Adv., 2019,9, 35820-35824
Article type
Paper

Bulky cations greatly increase the turnover of a native hammerhead ribozyme

S. Nakano, H. Yamashita, K. Tanabe and N. Sugimoto, RSC Adv., 2019, 9, 35820
DOI: 10.1039/C9RA06797C

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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