Jump to main content
Jump to site search

Issue 48, 2019, Issue in Progress
Previous Article Next Article

Screening of multi-class antibiotics in pork meat by LC-Orbitrap-MS with modified QuEChERS extraction

Author affiliations

Abstract

The quantification capability of high resolution mass spectrometry is of great interest to analysts. We described a method for analysis of multi-class antibiotics in pork meat by UPLC-quadrupole (Q)-Orbitrap-MS. The QuEChERS approach with a clean-up step using a sorbent of primary-secondary amine (PSA) and C18 was adopted for sample preparation, and 37 antibiotics including beta-lactams, tetracyclines, sulfonamides, fluoroquinolones and macrolides were analyzed. The Q-Orbitrap method showed high sensitivity with limits of detection (LODs) ranging from 0.8 μg kg−1 to 2.9 μg kg−1. The method was further validated by intra and inter-day tests with fortified samples. Recovery (85–105.6%) and precision values (RSDs < 15%) for all analytes were obtained. The result indicates that UPLC-Q-Orbitrap-MS coupled with QuEChERS preparation can serve as a routine method for multi-class antibiotic analysis in pork meat.

Graphical abstract: Screening of multi-class antibiotics in pork meat by LC-Orbitrap-MS with modified QuEChERS extraction

Back to tab navigation

Publication details

The article was received on 27 Jun 2019, accepted on 27 Aug 2019 and first published on 06 Sep 2019


Article type: Paper
DOI: 10.1039/C9RA04853G
RSC Adv., 2019,9, 28119-28125
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Screening of multi-class antibiotics in pork meat by LC-Orbitrap-MS with modified QuEChERS extraction

    Q. Chen, X. Pan, B. Huang, J. Han and B. Zhou, RSC Adv., 2019, 9, 28119
    DOI: 10.1039/C9RA04853G

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements