Issue 47, 2019, Issue in Progress

Signal amplification method for miR-205 assay through combining graphene oxide with duplex-specific nuclease

Abstract

Since microRNA-205 (miR-205) is a predictive biomarker for anti-radiation of nasopharyngeal carcinoma (NPC), quantitative detection of miR-205 is important for developing personalized strategies for the treatment of NPC. In this investigation, based on the graphene oxide sensor and duplex specific nuclease (DSN) for fluorescence signal amplification, a highly sensitive detection method for miR-205 was designed. A target-recycling mechanism is employed, where a single miR-205 target triggers the cleavage of many DNA signal probes. The method shows the ability to analyze miR-205 in solution, and it can detect miR-205 at concentrations as low as 132 pmol L−1 with a linear range of 5–40 nmol L−1. Furthermore, the method is specific in that it distinguishes between a target miRNA and a sequence with single base, double base and three base mismatches, as well as other miRNAs. Considering simplicity and excellent sensitivity/specificity, it is promising for applications in radioresistance studies as well as the early clinical diagnosis of NPC.

Graphical abstract: Signal amplification method for miR-205 assay through combining graphene oxide with duplex-specific nuclease

Article information

Article type
Paper
Submitted
21 Jun 2019
Accepted
27 Aug 2019
First published
30 Aug 2019
This article is Open Access
Creative Commons BY license

RSC Adv., 2019,9, 27341-27346

Signal amplification method for miR-205 assay through combining graphene oxide with duplex-specific nuclease

Z. Yang, L. Qin, D. Yang, W. Chen, Y. Qian and J. Jin, RSC Adv., 2019, 9, 27341 DOI: 10.1039/C9RA04663A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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