Issue 62, 2019

CSSF-CLIP-HSQMBC: measurement of heteronuclear coupling constants in severely crowded spectral regions

Abstract

A new pulse program development, a chemical shift selective filtration clean in-phase HSQMBC (CSSF-CLIP-HSQMBC), is presented for the user-friendly measurement of long-range heteronuclear coupling constants in severely crowded spectral regions. The introduction of the chemical shift selective filter makes the experiment extremely efficient at resolving overlapped multiplets and produces a clean selective CLIP-HSQMBC spectrum, in which the desired coupling constants can easily be measured as an extra proton–carbon splitting in f2. The pulse sequence is also provided as a real-time homonuclear decoupled version in which the heteronuclear coupling constant can be directly measured as the peak splitting in f2. The same principle is readily applicable to IPAP and AP versions of the same sequence as well as the optional TOCSY transfer, or in principle to any other selective heteronuclear experiment that relies on a clean 1H multiplet.

Graphical abstract: CSSF-CLIP-HSQMBC: measurement of heteronuclear coupling constants in severely crowded spectral regions

Supplementary files

Article information

Article type
Paper
Submitted
31 May 2019
Accepted
18 Oct 2019
First published
05 Nov 2019
This article is Open Access
Creative Commons BY license

RSC Adv., 2019,9, 36082-36087

CSSF-CLIP-HSQMBC: measurement of heteronuclear coupling constants in severely crowded spectral regions

A. Moreno, K. Ø. Hansen and J. Isaksson, RSC Adv., 2019, 9, 36082 DOI: 10.1039/C9RA04118D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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