Monooxygenases exhibiting high activity and differing regioselectivity for the dietary isoflavone metabolite equol were discovered among enzymes in the HpaBC family by a genome mining approach. These enzymes enabled the one-step product-selective synthesis of 3′- and 6-hydroxyequols from equol and molecular oxygen.
T. Hashimoto, D. Nozawa, K. Mukai, A. Matsuyama, K. Kuramochi and T. Furuya, RSC Adv., 2019, 9, 21826 DOI: 10.1039/C9RA03913A
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