Issue 32, 2019

Highly malleable haem-binding site of the haemoprotein HasA permits stable accommodation of bulky tetraphenylporphycenes

Abstract

Iron(III)- and cobalt(III)-9,10,19,20-tetraphenylporphycenes, which possess bulky phenyl groups at the four meso positions of porphycene, were successfully incorporated into the haem acquisition protein HasA secreted by Pseudomonas aeruginosa. Crystal structure analysis revealed that loops surrounding the haem-binding site are highly flexible, remodelling themselves to accommodate bulky metal complexes with significantly different structures from the native haem cofactor.

Graphical abstract: Highly malleable haem-binding site of the haemoprotein HasA permits stable accommodation of bulky tetraphenylporphycenes

Supplementary files

Article information

Article type
Paper
Submitted
16 Apr 2019
Accepted
23 May 2019
First published
13 Jun 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 18697-18702

Highly malleable haem-binding site of the haemoprotein HasA permits stable accommodation of bulky tetraphenylporphycenes

E. Sakakibara, Y. Shisaka, H. Onoda, D. Koga, N. Xu, T. Ono, Y. Hisaeda, H. Sugimoto, Y. Shiro, Y. Watanabe and O. Shoji, RSC Adv., 2019, 9, 18697 DOI: 10.1039/C9RA02872B

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