Issue 43, 2019, Issue in Progress

Oxidative stress generated at nickel oxide nanoparticle interface results in bacterial membrane damage leading to cell death

Abstract

Metal oxide nanoparticles (NPs) have shown enhanced antibacterial effects against many bacteria. Thus, understanding the potential antibacterial effects of nickel oxide nanoparticles (NiO NPs) against Gram-positive and Gram-negative pathogenic bacteria is an urgent need to enable the exploration of NiO NP use in biomedical sciences. To this end, NiO NPs were synthesized by microwave assisted hydrothermal synthesis method. The synthesized NPs were characterized by X-ray diffraction (XRD) and Fourier Transfer Infrared (FT-IR) and UV-visible spectroscopy. The morphological features of the synthesized NiO NPs were analysed using Transmission Electron Microscopy (TEM) and FE-SEM analysis. The antibacterial activity of NiO NP was explored using different antimicrobial and biophysical studies. The obtained data reveals that the NiO NP has stronger antibacterial activity against Gram-positive bacteria compared to Gram-negative bacteria. The mechanism behind the antibacterial activity of the NiO NP was explored by evaluating the amount of ROS generation at the NiO NP interface. The effect of ROS generation on the bacterial membrane was evaluated by BacLight assay and morphological analysis of the bacterial membrane using FE-SEM. The data altogether suggested that the oxidative stress generated at the NiO NP interface resulted in membrane damage leading to bacterial cell death.

Graphical abstract: Oxidative stress generated at nickel oxide nanoparticle interface results in bacterial membrane damage leading to cell death

Supplementary files

Article information

Article type
Paper
Submitted
18 Mar 2019
Accepted
31 Jul 2019
First published
12 Aug 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 24888-24894

Oxidative stress generated at nickel oxide nanoparticle interface results in bacterial membrane damage leading to cell death

N. Behera, M. Arakha, M. Priyadarshinee, B. S. Pattanayak, S. Soren, S. Jha and B. C. Mallick, RSC Adv., 2019, 9, 24888 DOI: 10.1039/C9RA02082A

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