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Issue 18, 2019, Issue in Progress
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Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes

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Abstract

High numbers of autologous human primary keratinocytes (HPKs) are required for patients with burns, wounds and for gene therapy of skin disorders. Although freshly isolated HPKs exhibit a robust regenerative capacity, traditional methodology fails to provide a sufficient number of cells. Here we demonstrated a well characterized, non-cytotoxic and inert hydrogel as a substrate that mimics skin elasticity, which can accelerate proliferation and generate higher numbers of HPKs compared to existing tissue culture plastic (TCP) dishes. More importantly, this novel method was independent of feeder layer or any exogenous pharmaceutical drug. The HPKs from the hydrogel-substrate were functional as demonstrated by wound-healing assay, and the expression of IFN-γ-responsive genes (CXCL10, HLADR). Importantly, gene delivery efficiency by a lentiviral based delivery system was significantly higher in HPKs cultured on hydrogels compared with TCP. In conclusion, our study provides the first evidence that cell-material mechanical interaction is enough to provide a rapid expansion of functional keratinocytes that might be used as autologous grafts for skin disorders.

Graphical abstract: Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes

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Supplementary files

Article information


Submitted
29 Jan 2019
Accepted
13 Mar 2019
First published
01 Apr 2019

This article is Open Access

RSC Adv., 2019,9, 10174-10183
Article type
Paper

Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes

P. Mogha, A. Srivastava, S. Kumar, S. Das, S. Kureel, A. Dwivedi, A. Karulkar, N. Jain, A. Sawant, C. Nayak, A. Majumder and R. Purwar, RSC Adv., 2019, 9, 10174
DOI: 10.1039/C9RA00781D

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