One step DNA amplification of mammalian cells in picoliter microwell arrays

Wenwen Liu; Zhao Li; Yuanjie Liu; Qingquan Wei; Yong Liu; Lufeng Ren; Chenyu Wang; Yude Yu

doi:10.1039/C8RA06717A

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One step DNA amplification of mammalian cells in picoliter microwell arrays†

Wenwen Liu,

^a Zhao Li,^a Yuanjie Liu,^a Qingquan Wei,^a Yong Liu,^a Lufeng Ren,^a Chenyu Wang

^a and Yude Yu*^a

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* Corresponding authors

^a State Key Laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, P. O. Box 912, Beijing, China
E-mail: yudeyu@semi.ac.cn

Abstract

We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30 600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy.

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Article information

DOI: https://doi.org/10.1039/C8RA06717A
Article type: Paper
Submitted: 10 Aug 2018
Accepted: 23 Dec 2018
First published: 21 Jan 2019
This article is Open Access

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RSC Adv., 2019,9, 2865-2869

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One step DNA amplification of mammalian cells in picoliter microwell arrays

W. Liu, Z. Li, Y. Liu, Q. Wei, Y. Liu, L. Ren, C. Wang and Y. Yu, RSC Adv., 2019, 9, 2865 DOI: 10.1039/C8RA06717A

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One step DNA amplification of mammalian cells in picoliter microwell arrays†

Abstract

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One step DNA amplification of mammalian cells in picoliter microwell arrays

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