Issue 5, 2019
From the journal:

RSC Advances

One step DNA amplification of mammalian cells in picoliter microwell arrays†

Wenwen Liu, ORCID logo a   Zhao Li,a   Yuanjie Liu,a   Qingquan Wei,a   Yong Liu,a   Lufeng Ren,a   Chenyu Wang ORCID logo a  and  Yude Yu*a  

* Corresponding authors

a State Key Laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, P. O. Box 912, Beijing, China
E-mail: yudeyu@semi.ac.cn

Abstract

We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30 600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy.

Graphical abstract: One step DNA amplification of mammalian cells in picoliter microwell arrays

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Article information

DOI
https://doi.org/10.1039/C8RA06717A
Article type
Paper
Submitted
10 Aug 2018
Accepted
23 Dec 2018
First published
21 Jan 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 2865-2869

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One step DNA amplification of mammalian cells in picoliter microwell arrays

W. Liu, Z. Li, Y. Liu, Q. Wei, Y. Liu, L. Ren, C. Wang and Y. Yu, RSC Adv., 2019, 9, 2865 DOI: 10.1039/C8RA06717A

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