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Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles

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Abstract

Antifreeze proteins (AFPs) have many potential applications, ranging from cryobiology to aerospace, if they can be incorporated into materials. Here, a range of engineered AFP mutants were prepared and site-specifically conjugated onto RAFT polymer-stabilized gold nanoparticles to generate new hybrid multivalent ice growth inhibitors. Only the SNAP-tagged AFPs lead to potent ‘antifreeze’ active nanomaterials with His-Tag capture resulting in no activity, showing the mode of conjugation is essential. This versatile strategy will enable the development of multivalent AFPs for translational and fundamental studies.

Graphical abstract: Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles

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Publication details

The article was received on 03 Dec 2018, accepted on 21 Jan 2019 and first published on 31 Jan 2019


Article type: Communication
DOI: 10.1039/C8PY01719K
Citation: Polym. Chem., 2019, Advance Article
  • Open access: Creative Commons BY license
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    Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles

    L. E. Wilkins, M. Hasan, A. E. R. Fayter, C. Biggs, M. Walker and M. I. Gibson, Polym. Chem., 2019, Advance Article , DOI: 10.1039/C8PY01719K

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