NIR Fluorescent Probe for Detection of Viscosity and Lysosome Imaging in Live Cells
Lysosome, as the cellular recycling center, is filled with numerous hydrolases that can degrade most cellular macromolecules. Studies have shown that the abnormality of viscosity in lysosome will disorder the normal function of lysosome. Herein, a D-π-A structure near-infrared fluorescent probe containing N, N-dimethyamino benzene as electron donor, benzothiozole as electron acceptor, and vinyl group as π unit, Lyso-BTC, is explored for fluorescent imaging of lysosome and detection of lysosomal viscosity change. Lyso-BTC exhibits large Stokes shift (~180 nm), NIR emission (685 nm), good biocompatibility, excellent photostability, and fluorescent response to viscosity. Moreover, the results of in vitro studies reveal that Lyso-BTC is lysosome-targeted and could be applied for the detection of viscosity change in lysosome caused by chloroquine treatment. These results confirm that Lys-BTC could be employed to monitor lysosomal viscosity change in the living cells.
- This article is part of the themed collection: Chemical biology in OBC