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Issue 20, 2019
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TuPPL: Tub-tag mediated C-terminal protein–protein-ligation using complementary click-chemistry handles

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Abstract

We introduce a chemoenzymatic strategy for straightforward in vitro generation of C-terminally linked fusion proteins. Tubulin tyrosine ligase is used for the incorporation of complementary click chemistry handles facilitating subsequent formation of functional bispecific antibody-fragments. This simple strategy may serve as central conjugation hub for a modular protein ligation platform.

Graphical abstract: TuPPL: Tub-tag mediated C-terminal protein–protein-ligation using complementary click-chemistry handles

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Article information


Submitted
01 Mar 2019
Accepted
05 Mar 2019
First published
01 Apr 2019

This article is Open Access

Org. Biomol. Chem., 2019,17, 4964-4969
Article type
Communication

TuPPL: Tub-tag mediated C-terminal protein–protein-ligation using complementary click-chemistry handles

A. Stengl, M. Gerlach, M. Kasper, C. P. R. Hackenberger, H. Leonhardt, D. Schumacher and J. Helma, Org. Biomol. Chem., 2019, 17, 4964
DOI: 10.1039/C9OB00508K

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