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Issue 6, 2019
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Imidazole carbamate probes for affinity guided azide-transfer to metal-binding proteins

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Abstract

Probes for affinity guided conjugation have shown great promise for the preparation of high-quality protein conjugates. However, such probes are often limited in their protein scope. Here, we demonstrate the synthesis and use of imidazole carbamate probes for affinity guided conjugation of an azide handle to a wide variety of metal binding proteins such as antibodies, enzymes, nanobodies and His6-tagged proteins. The azide handles were used to link dyes to the proteins for gel analysis and binding studies. In a comparative study we find that the probe containing three NTA groups show higher selectivity than the probe containing two. In a mixture of proteins, the probe shows preference for labelling His6-tagged proteins.

Graphical abstract: Imidazole carbamate probes for affinity guided azide-transfer to metal-binding proteins

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Publication details

The article was received on 04 Dec 2018, accepted on 10 Jan 2019 and first published on 16 Jan 2019


Article type: Communication
DOI: 10.1039/C8OB03017K
Citation: Org. Biomol. Chem., 2019,17, 1379-1383

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    Imidazole carbamate probes for affinity guided azide-transfer to metal-binding proteins

    M. R. Mortensen, N. L. Nielsen, J. Palmfeldt and K. V. Gothelf, Org. Biomol. Chem., 2019, 17, 1379
    DOI: 10.1039/C8OB03017K

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