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Issue 7, 2019
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Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

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Abstract

The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.

Graphical abstract: Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

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Publication details

The article was received on 19 Oct 2018, accepted on 27 Nov 2018 and first published on 28 Nov 2018


Article type: Communication
DOI: 10.1039/C8OB02597E
Citation: Org. Biomol. Chem., 2019,17, 1805-1808

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    Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

    C. Domnick, G. Hagelueken, F. Eggert, O. Schiemann and S. Kath-Schorr, Org. Biomol. Chem., 2019, 17, 1805
    DOI: 10.1039/C8OB02597E

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