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Issue 37, 2019
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A non-cationic nucleic acid nanogel for the delivery of the CRISPR/Cas9 gene editing tool

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Abstract

Herein, we report a non-cationic DNA-crosslinked nanogel for intracellular delivery of a Cas9 and single guide RNA (Cas9/sgRNA) complex. A DNA-grafted polycaprolactone brush (DNA-g-PCL) is first loaded with the Cas9/sgRNA complex and then crosslinked by DNA linkers via nucleic acid hybridization to form a nanosized hydrogel, in which the gene editing tools are embedded and protected inside. With compact architecture, the Cas9/sgRNA complex-containing nanogel exhibited excellent physiological stability against nuclease digestion and enhanced cellular uptake efficiency, making the delivery system a promising tool for target genome editing.

Graphical abstract: A non-cationic nucleic acid nanogel for the delivery of the CRISPR/Cas9 gene editing tool

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Supplementary files

Article information


Submitted
20 Jun 2019
Accepted
27 Aug 2019
First published
28 Aug 2019

Nanoscale, 2019,11, 17211-17215
Article type
Communication

A non-cationic nucleic acid nanogel for the delivery of the CRISPR/Cas9 gene editing tool

F. Ding, X. Huang, X. Gao, M. Xie, G. Pan, Q. Li, J. Song, X. Zhu and C. Zhang, Nanoscale, 2019, 11, 17211
DOI: 10.1039/C9NR05233J

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