Jump to main content
Jump to site search

Issue 2, 2019
Previous Article Next Article

A new DNA sensor system for specific and quantitative detection of mycobacteria

Author affiliations


In the current study, we describe a novel DNA sensor system for specific and quantitative detection of mycobacteria, which is the causative agent of tuberculosis. Detection is achieved by using the enzymatic activity of the mycobacterial encoded enzyme topoisomerase IA (TOP1A) as a biomarker. The presented work is the first to describe how the catalytic activities of a member of the type IA family of topoisomerases can be exploited for specific detection of bacteria. The principle for detection relies on a solid support anchored DNA substrate with dual functions namely: (1) the ability to isolate mycobacterial TOP1A from crude samples and (2) the ability to be converted into a closed DNA circle upon reaction with the isolated enzyme. The DNA circle can act as a template for rolling circle amplification generating a tandem repeat product that can be visualized at the single molecule level by fluorescent labelling. This reaction scheme ensures specific, sensitive, and quantitative detection of the mycobacteria TOP1A biomarker as demonstrated by the use of purified mycobacterial TOP1A and extracts from an array of non-mycobacteria and mycobacteria species. When combined with mycobacteriophage induced lysis as a novel way of effective yet gentle extraction of the cellular content from the model Mycobacterium smegmatis, the DNA sensor system allowed detection of mycobacteria in small volumes of cell suspensions. Moreover, it was possible to detect M. smegmatis added to human saliva. Depending on the composition of the sample, we were able to detect 0.6 or 0.9 million colony forming units (CFU) per mL of mycobacteria, which is within the range of clinically relevant infection numbers. We, therefore, believe that the presented assay, which relies on techniques that can be adapted to limited resource settings, may be the first step towards the development of a new point-of-care diagnostic test for tuberculosis.

Graphical abstract: A new DNA sensor system for specific and quantitative detection of mycobacteria

Back to tab navigation

Supplementary files

Publication details

The article was received on 27 Sep 2018, accepted on 25 Nov 2018 and first published on 28 Nov 2018

Article type: Paper
DOI: 10.1039/C8NR07850E
Citation: Nanoscale, 2019,11, 587-597

  •   Request permissions

    A new DNA sensor system for specific and quantitative detection of mycobacteria

    O. Franch, X. Han, L. B. Marcussen, A. Givskov, M. B. Andersen, A. A. Godbole, C. Harmsen, N. Nørskov-Lauritsen, J. Thomsen, F. S. Pedersen, Y. Wang, D. Shi, C. Wejse, L. Pødenphant, V. Nagaraja, J. Bertl, M. Stougaard, Y. Ho, M. S. Hede, R. Labouriau and B. R. Knudsen, Nanoscale, 2019, 11, 587
    DOI: 10.1039/C8NR07850E

Search articles by author