Jump to main content
Jump to site search

Issue 44, 2019
Previous Article Next Article

An electrically and magnetically responsive nanocomposite of GdPO4·H2O/P3HT/PLGA with electrical stimulation for synergistically enhancing the proliferation and differentiation of pre-osteoblasts

Author affiliations

Abstract

Although electroactive and magnetic biomedical composites have been proven respectively to promote bone regeneration, their associated biological effects have not been investigated. Herein, electroactive GdPO4·H2O/P3HT/PLGA nanocomposites were prepared, which can enhance MRI and CT signals with different GdPO4·H2O content (0.0, 1.0, 3.5, or 7.0 wt%). The morphology, hydrophilicity, magnetism, electroactivity and conductivity of the composites were characterized. Compared with PLGA, the composites exhibit good paramagnetism and electroactivity. The cell morphology and viability of pre-osteoblasts (MC3T3-E1 cell), their alkaline phosphatase (ALP) activity, calcium deposition, relative expression level of osteogenic genes, and MRI and CT imaging were studied. The proliferation of pre-osteoblasts was obviously increased on the GdPO4·H2O (3.5 wt%)/P3HT/PLGA composite, and the composite containing 7.0 wt% GdPO4·H2O significantly promoted osteogenic differentiation by increasing the ALP activity and calcium deposition, as well as the gene expression of Runx 2, OPN and OCN. Moreover, an enhanced effect was observed in osteogenic differentiation when the electroactive composites were coupled with electrical stimulation (ES) at a frequency of 100 Hz and electrical potential of 500 mV. In particular, OPN expression with GdPO4·H2O (7.0 wt%) was up-regulated 3.0-fold compared to on glass with ES after 7 days. Besides this, the MRI and CT signals gradually enhanced with increasing GdPO4·H2O content. Overall, the electroactive nanocomposite with traceability combined with ES can synergistically up-regulate cellular activity and may be a promising bone implant for bone repair and regeneration.

Graphical abstract: An electrically and magnetically responsive nanocomposite of GdPO4·H2O/P3HT/PLGA with electrical stimulation for synergistically enhancing the proliferation and differentiation of pre-osteoblasts

Back to tab navigation

Supplementary files

Article information


Submitted
13 Aug 2019
Accepted
11 Oct 2019
First published
14 Oct 2019

New J. Chem., 2019,43, 17315-17326
Article type
Paper

An electrically and magnetically responsive nanocomposite of GdPO4·H2O/P3HT/PLGA with electrical stimulation for synergistically enhancing the proliferation and differentiation of pre-osteoblasts

H. Yan, L. Li, Y. Wang, J. Huang, Z. Wang, X. Shi and P. Zhang, New J. Chem., 2019, 43, 17315
DOI: 10.1039/C9NJ04167B

Social activity

Search articles by author

Spotlight

Advertisements