A ratiometric fluorescent chemosensor for the convenient monitoring of hydrogen sulfide concentration by the dual fluorescence fluctuation mode of two distinct emission bands in living cells and zebrafish

Abstract

Herein, an interplaying ICT-FRET-based ratiometric fluorescent chemosensor (NapN₃–PCM), capable of precisely determining H₂S concentration by the dual fluorescence fluctuation mode of two distinct emission bands, was elaborately designed and synthesized. Importantly, it was successfully applied to detect H₂S levels in living cells and zebrafish using ratiometric fluorescence imaging.