Target exploration of rhein as a small-molecule necrosis avid agent by post-treatment click modification

Abstract

Tracking of necrosis avid agents is of crucial importance toward understanding their mechanisms. We explored the cellular localisation of rhein by synthesising a clickable derivative (compound 3) and labelling it in situ with an Alexa-fluor 488 click partner. Binding to exposed DNA may be an important mechanism of targeting of rhein compounds to necrotic cells.