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pH-Dependent fluorescence from firefly oxyluciferin in agarose thin films

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Abstract

The yellow-green light emitted by fireflies is one of the most prominent examples of bioluminescence. Firefly oxyluciferin, the emitting molecule, is labile in alkaline solutions, and its structure is strongly affected by solvent polarity and pH. Previous studies have suggested that variations in the active site conditions are likely contributors to the color of bioluminescent emission. Herein, we incorporate firefly oxyluciferin into an agarose matrix to emulate the enzyme active site. Self-supporting, lightweight thin films were fabricated by solution casting and spectroscopically characterized. The previously described acidochromism of oxyluciferin is conserved in the thin films. The bathochromic shift observed in alkaline conditions results from the formation of the oxyluciferin dianion. This study demonstrates an alternative approach to investigating environmental effects on bioluminescent molecules.

Graphical abstract: pH-Dependent fluorescence from firefly oxyluciferin in agarose thin films

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Publication details

The article was received on 28 Oct 2018, accepted on 26 Nov 2018 and first published on 27 Nov 2018


Article type: Letter
DOI: 10.1039/C8NJ05469J
Citation: New J. Chem., 2019, Advance Article
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    pH-Dependent fluorescence from firefly oxyluciferin in agarose thin films

    N. M. Lui, S. Schramm and P. Naumov, New J. Chem., 2019, Advance Article , DOI: 10.1039/C8NJ05469J

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