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Issue 14, 2019
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Electronic profiling of membrane antigen expression via immunomagnetic cell manipulation

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Abstract

Membrane antigens control cell function by regulating biochemical interactions and hence are routinely used as diagnostic and prognostic targets in biomedicine. Fluorescent labeling and subsequent optical interrogation of cell membrane antigens, while highly effective, limit expression profiling to centralized facilities that can afford and operate complex instrumentation. Here, we introduce a cytometry technique that computes surface expression of immunomagnetically labeled cells by electrically tracking their trajectory under a magnetic field gradient on a microfluidic chip with a throughput of >500 cells per min. In addition to enabling the creation of a frugal cytometry platform, this immunomagnetic cell manipulation-based measurement approach allows direct expression profiling of target subpopulations from non-purified samples. We applied our technology to measure epithelial cell adhesion molecule expression on human breast cancer cells. Once calibrated, surface expression and size measurements match remarkably well with fluorescence-based measurements from a commercial flow cytometer. Quantitative measurements of biochemical and biophysical cell characteristics with a disposable cytometer have the potential to impact point of care testing of clinical samples particularly in resource limited settings.

Graphical abstract: Electronic profiling of membrane antigen expression via immunomagnetic cell manipulation

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Publication details

The article was received on 31 Mar 2019, accepted on 22 May 2019 and first published on 14 Jun 2019


Article type: Paper
DOI: 10.1039/C9LC00297A
Lab Chip, 2019,19, 2444-2455
  • Open access: Creative Commons BY-NC license
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    Electronic profiling of membrane antigen expression via immunomagnetic cell manipulation

    O. Civelekoglu, N. Wang, M. Boya, T. Ozkaya-Ahmadov, R. Liu and A. F. Sarioglu, Lab Chip, 2019, 19, 2444
    DOI: 10.1039/C9LC00297A

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