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Issue 13, 2019
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Two-dimensional planar swimming selects for high DNA integrity sperm

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Abstract

Selection of high-quality sperm is critical to the success of assisted reproductive technologies. Clinical screening for top sperm has long focused on sperm swimming ability when following boundaries or when fully free of constraints. In this work, we demonstrate a sperm selection approach with parallel 2 μm tall confined selection channels that prohibit rotation of the sperm head and require planar swimming. We demonstrate that a planar swimming subpopulation of sperm capable of entering and navigating these channels has DNA integrity superior to the freely-swimming motile or raw sperm populations over a wide range of semen sample qualities. The DNA integrity of the selected sperm was significantly higher than that of the corresponding raw samples for donor samples and clinical patient samples, respectively. In side-by-side testing, this method outperforms current clinical selection methods, density gradient centrifugation and swim-up, as well as sperm selected via general motility. Planar swimming represents a viable sperm selection mechanism with the potential to improve outcomes for couples and offspring.

Graphical abstract: Two-dimensional planar swimming selects for high DNA integrity sperm

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Publication details

The article was received on 27 Feb 2019, accepted on 08 May 2019 and first published on 08 May 2019


Article type: Paper
DOI: 10.1039/C9LC00209J
Lab Chip, 2019,19, 2161-2167

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    Two-dimensional planar swimming selects for high DNA integrity sperm

    J. Riordon, F. Tarlan, J. B. You, B. Zhang, P. J. Graham, T. Kong, Y. Wang, A. Lagunov, T. Hannam, K. Jarvi and D. Sinton, Lab Chip, 2019, 19, 2161
    DOI: 10.1039/C9LC00209J

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