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A microfluidic platform with cell-scale precise temperature control for simultaneous investigation of the osmotic responses of multiple oocytes

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Abstract

The temperature-dependent oocyte membrane permeability plays a significant role in oocyte cryopreservation, such as optimizing the addition/removal of cryoprotective agents and the rate of cooling/rewarming. However, the systems for studying the temperature dependence of oocyte membrane permeability are either too complicated or unable to achieve wide-range precise temperature control. In addition, these systems cannot achieve the simultaneous observation of multiple oocytes. Here, we report a novel microfluidic platform that combines a precise local temperature heater/detector and a simple global water bath to achieve wide-range accurate temperature control without increasing the difficulty of fabrication, and it also realizes non-interfering, position-controllable and non-missing capture of multiple oocytes for parallel experiments to increase throughput. The permeability coefficients (Lp, Ps) of the mouse oocyte membrane exposed to cryoprotective agents (1.5 M EG and 1.5 M PG) at four temperatures (4, 15, 25 and 37 °C) are consistent with those reported in previous works, which proves the feasibility and practicality of the microfluidic platform in this study.

Graphical abstract: A microfluidic platform with cell-scale precise temperature control for simultaneous investigation of the osmotic responses of multiple oocytes

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Publication details

The article was received on 31 Jan 2019, accepted on 13 Apr 2019 and first published on 16 Apr 2019


Article type: Paper
DOI: 10.1039/C9LC00107G
Lab Chip, 2019, Advance Article

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    A microfluidic platform with cell-scale precise temperature control for simultaneous investigation of the osmotic responses of multiple oocytes

    Z. Lei, D. Xie, M. K. Mbogba, Z. Chen, C. Tian, L. Xu and G. Zhao, Lab Chip, 2019, Advance Article , DOI: 10.1039/C9LC00107G

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