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Issue 7, 2019
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Lab-on-a-Film disposable for genotyping multidrug-resistant Mycobacterium tuberculosis from sputum extracts

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Abstract

We describe a Lab-on-a-Film disposable that detects multidrug-resistant tuberculosis (MDR-TB) from sputum extracts. The Lab-on-a-Film disposable consists of 203 gel elements that include DNA sequences (probes) for 37 mutations, deletions, or insertion elements across 5 genes (including an internal control). These gel elements are printed on a flexible film, which costs approximately 500 times less than microarray glass. The film with printed gel elements is then laminated to additional rollable materials (films) to form a microfluidic flow cell. We combined multiplex amplification and hybridization steps in a single microfluidic chamber, without buffer exchanges or other manipulations up to and throughout hybridization. This flow cell also incorporates post hybridization wash steps while retaining an entirely closed-amplicon system, thus minimizing the potential for sample or amplicon cross-contamination. We report analytical sensitivity of 32 cfu mL−1 across all MDR-TB markers and detection of MDR-TB positive clinical specimens using an automated TruTip workstation for extraction and the Lab-on-a-Film disposable for amplification and detection of the extracts.

Graphical abstract: Lab-on-a-Film disposable for genotyping multidrug-resistant Mycobacterium tuberculosis from sputum extracts

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Publication details

The article was received on 24 Dec 2018, accepted on 12 Feb 2019 and first published on 15 Feb 2019


Article type: Paper
DOI: 10.1039/C8LC01404C
Lab Chip, 2019,19, 1217-1225

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    Lab-on-a-Film disposable for genotyping multidrug-resistant Mycobacterium tuberculosis from sputum extracts

    A. C. Kukhtin, T. Sebastian, J. Golova, A. Perov, C. Knickerbocker, Y. Linger, A. Bueno, P. Qu, M. Villanueva, R. C. Holmberg, D. P. Chandler and C. G. Cooney, Lab Chip, 2019, 19, 1217
    DOI: 10.1039/C8LC01404C

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