Jump to main content
Jump to site search


High-throughput, non-equilibrium studies of single biomolecules using glass-made nanofluidic devices

Author affiliations

Abstract

Single-molecule detection schemes offer powerful means to overcome static and dynamic heterogeneity inherent to complex samples. However, probing biomolecular interactions and reactions with high throughput and time resolution remains challenging, often requiring surface-immobilized entities. Here, we introduce glass-made nanofluidic devices for the high-throughput detection of freely-diffusing single biomolecules by camera-based fluorescence microscopy. Nanochannels of 200 nm height and a width of several micrometers confine the movement of biomolecules. Using pressure-driven flow through an array of parallel nanochannels and by tracking the movement of fluorescently labelled DNA oligonucleotides, we observe conformational changes with high throughput. In a device geometry featuring a T-shaped junction of nanochannels, we drive steady-state non-equilibrium conditions by continuously mixing reactants and triggering chemical reactions. We use the device to probe the conformational equilibrium of a DNA hairpin as well as to continuously observe DNA synthesis in real time. Our platform offers a straightforward and robust method for studying reaction kinetics at the single-molecule level.

Graphical abstract: High-throughput, non-equilibrium studies of single biomolecules using glass-made nanofluidic devices

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Oct 2018, accepted on 20 Nov 2018 and first published on 20 Nov 2018


Article type: Paper
DOI: 10.1039/C8LC01175C
Citation: Lab Chip, 2019, Advance Article
  •   Request permissions

    High-throughput, non-equilibrium studies of single biomolecules using glass-made nanofluidic devices

    M. Fontana, C. Fijen, S. G. Lemay, K. Mathwig and J. Hohlbein, Lab Chip, 2019, Advance Article , DOI: 10.1039/C8LC01175C

Search articles by author

Spotlight

Advertisements