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Issue 2, 2019
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Flow focusing through gels as a tool to generate 3D concentration profiles in hydrogel-filled microfluidic chips

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Abstract

Laminar flow patterning is an iconic microfluidic technology used to deliver chemicals to specific regions on a two-dimensional surface with high spatial fidelity. Here we present a novel extension of this technology using Darcy flow within a three-dimensional (3D) hydrogel. Our test device is a simple 3-inlet microfluidic channel, totally filled with collagen, a cured biological hydrogel, where the concentration profiles of solutes are manipulated via the inlet pressures. This method allows solutes to be delivered with 50 micron accuracy within the gel, as we evidence by controlling concentration profiles of 40 kDa and 1 kDa fluorescent polysaccharide dyes. Furthermore, we design and test a 3D-printed version of our device with an extra two inlets for control of the vertical position of the concentration profile, demonstrating that this method is easily extensible to control of the concentration profile in 3D.

Graphical abstract: Flow focusing through gels as a tool to generate 3D concentration profiles in hydrogel-filled microfluidic chips

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Publication details

The article was received on 23 Oct 2018, accepted on 09 Nov 2018 and first published on 14 Dec 2018


Article type: Communication
DOI: 10.1039/C8LC01140K
Citation: Lab Chip, 2019,19, 206-213
  • Open access: Creative Commons BY license
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    Flow focusing through gels as a tool to generate 3D concentration profiles in hydrogel-filled microfluidic chips

    J. Loessberg-Zahl, A. D. van der Meer, A. van den Berg and J. C. T. Eijkel, Lab Chip, 2019, 19, 206
    DOI: 10.1039/C8LC01140K

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