Jump to main content
Jump to site search

Issue 1, 2019
Previous Article Next Article

Resolving protein mixtures using microfluidic diffusional sizing combined with synchrotron radiation circular dichroism

Author affiliations

Abstract

Circular dichroism spectroscopy has become a powerful tool to characterise proteins and other biomolecules. For heterogeneous samples such as those present for interacting proteins, typically only average spectroscopic features can be resolved. Here we overcome this limitation by using free-flow microfluidic size separation in-line with synchrotron radiation circular dichroism to resolve the secondary structure of each component of a model protein mixture containing monomers and fibrils. To enable this objective, we have integrated far-UV compatible measurement chambers into PDMS-based microfluidic devices. Two architectures are proposed so as to accommodate for a wide range of concentrations. The approach, which can be used in combination with other bulk measurement techniques, paves the way to the study of complex mixtures such as the ones associated with protein misfolding and aggregation diseases including Alzheimer's and Parkinson's diseases.

Graphical abstract: Resolving protein mixtures using microfluidic diffusional sizing combined with synchrotron radiation circular dichroism

Back to tab navigation

Supplementary files

Publication details

The article was received on 22 Jul 2018, accepted on 13 Nov 2018 and first published on 22 Nov 2018


Article type: Paper
DOI: 10.1039/C8LC00757H
Citation: Lab Chip, 2019,19, 50-58
  •   Request permissions

    Resolving protein mixtures using microfluidic diffusional sizing combined with synchrotron radiation circular dichroism

    C. Bortolini, T. Kartanas, D. Copic, I. Condado Morales, Y. Zhang, P. K. Challa, Q. Peter, T. Jávorfi, R. Hussain, M. Dong, G. Siligardi, T. P. J. Knowles and J. Charmet, Lab Chip, 2019, 19, 50
    DOI: 10.1039/C8LC00757H

Search articles by author

Spotlight

Advertisements