Jump to main content
Jump to site search


Wheat alkylresorcinols protect human retinal pigment epithelial cells against H2O2-induced oxidative damage through Akt-dependent Nrf2/HO-1 signaling

Abstract

The protective effect of wheat alkylresorcinols (ARs) on ARPE-19 cells against oxidative stress and the possible underlying mechanism were investigated in this study. The results showed that ARs significantly inhibited 300 μM H2O2-induced ARPE-19 cells damage and reactive oxygen species (ROS) generation by 19 % and 32 %, respectively. Moreover, ARs treatment increased NF-E2-related factor 2 (Nrf2) signaling activation, which was evidenced by increasing transcription of anti-oxidant responsive, GCL, NQO1 and HO-1. Knockdown of Nrf2 through targeted siRNA alleviated ARs-mediated HO-1 transcription, and almost abolished ARs-mediated cytoprotection against H2O2 induced cell damage. Further studies showed that the protective effect of ARs was depended on Akt activation. Taken together, these results demonstrated that ARs could protect ARPE-19 cells from oxidative stress induced cell damage possibly through Akt dependent Nrf2/HO-1 signaling.

Back to tab navigation

Publication details

The article was received on 25 Dec 2018, accepted on 12 Apr 2019 and first published on 15 Apr 2019


Article type: Paper
DOI: 10.1039/C8FO02564A
Citation: Food Funct., 2019, Accepted Manuscript

  •   Request permissions

    Wheat alkylresorcinols protect human retinal pigment epithelial cells against H2O2-induced oxidative damage through Akt-dependent Nrf2/HO-1 signaling

    Z. Wang, Y. Hao, Y. Wang, J. Liu, X. Yuan, B. Sun and J. Wang, Food Funct., 2019, Accepted Manuscript , DOI: 10.1039/C8FO02564A

Search articles by author

Spotlight

Advertisements