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Study on the structure and in vitro hypoglycemic activity of a homogenous polysaccharide purified from Sargassum pallidum

Abstract

This study aimed at investigating the structure, hypoglycemic activity and the underlying mechanism of a homogenous polysaccharide (PSP-2) purified from Sargassum pallidum. Structural characterization revealed that PSP-2 with the molecular weight of 144.8 kDa, was composed of fucose (21.6%), arabinose (2.5%), galactose (22.4%), glucose (2.2%), xylose (18.8%), mannose (1.2%), glucuronic acid (7.7%) and galacturonic acid (23.6%). The backbone chain of PSP-2 was composed of →1)-β-D-Xylp-(3→, →1,3)-β-L-Fucp-(4→, →1)-α-D-Galp-(6→, and →1)-α-D-GlcpNAc-(2→, and the side chains were composed of →1,3,6)-α-D-Galp-(2→, →3)-β-L-Fucp-(1,4→, β-D-GalpNAc-(1→, and α-D-Manp-(1→. In vitro hypoglycemic assays indicated that PSP-2 could significantly enhance glucose consumption, glycogen synthesis, and pyruvate kinase (PK) and hexokinase (HK) activities of the insulin-resistance HepG2 cells. Furthermore, the underlying mechanistic studies revealed that PSP-2 could ameliorate insulin resistance by up-regulating the expression levels of insulin receptor substrate-1 (IRS-1), glycogen synthase (GS), phosphoinositide-3-kinase (PI3K) and glucose transporter-4 (GLUT4). These results suggested that PSP-2 may be a potential candidate for prevention and treatment of Type 2 diabetes mellitus.

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Publication details

The article was received on 20 Dec 2018, accepted on 13 Apr 2019 and first published on 15 Apr 2019


Article type: Paper
DOI: 10.1039/C8FO02525H
Citation: Food Funct., 2019, Accepted Manuscript

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    Study on the structure and in vitro hypoglycemic activity of a homogenous polysaccharide purified from Sargassum pallidum

    C. Cao, B. Zhang, C. Li, Q. Huang, X. Fu and R. Liu, Food Funct., 2019, Accepted Manuscript , DOI: 10.1039/C8FO02525H

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