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Preliminary research on receptor-ligand recognition mechanism of umami by hT1R1 biosensor

Abstract

The aim of this work was to determine the interaction between human umami receptor hT1R1 and the ligand, at the same time to avoid "Cross-Talking" among various signal pathways in cells. The hT1R1 was modified and mounted to a signal amplification system on glassy carbon electrode surface, and the response current to four umami ligands (sodium glutamate (MSG), disodium inosinate (IMP), disodium guanylate (GMP), and disodium succinate (SUC)) was measured. The allosteric constants of receptor-ligand interaction were calculated by the method of sensing kinetics, and the results indicated that the ability of hT1R1 sensing to four ligands were as follows: GMP > MSG > IMP > SUC. After the analysis of molecular structure and simulation through molecular docking model, we found that hT1R1 was essentially a recognition receptor for “nitrogen” signal in the body, and it might recognize the umami substance through its amino group. The new research method developed in this paper showed promising application in the mechanism study of signal transduction and drug screening.

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Publication details

The article was received on 04 Jan 2019, accepted on 11 Feb 2019 and first published on 12 Feb 2019


Article type: Communication
DOI: 10.1039/C8FO02522C
Citation: Food Funct., 2019, Accepted Manuscript

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    Preliminary research on receptor-ligand recognition mechanism of umami by hT1R1 biosensor

    Y. Huang, D. Lu, H. Liu, S. Liu, S. Jiang, G. Pang and Y. Liu, Food Funct., 2019, Accepted Manuscript , DOI: 10.1039/C8FO02522C

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