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Fluorogenic probes for disease-relevant enzymes

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Abstract

Traditional biochemical methods for enzyme detection are mainly based on antibody-based immunoassays, which lack the ability to monitor the spatiotemporal distribution and, in particular, the in situ activity of enzymes in live cells and in vivo. In this review, we comprehensively summarize recent progress that has been made in the development of small-molecule as well as material-based fluorogenic probes for sensitive detection of the activities of enzymes that are related to a number of human diseases. The principles utilized to design these probes as well as their applications are reviewed. Specific attention is given to fluorogenic probes that have been developed for analysis of the activities of enzymes including oxidases and reductases, those that act on biomacromolecules including DNAs, proteins/peptides/amino acids, carbohydrates and lipids, and those that are responsible for translational modifications. We envision that this review will serve as an ideal reference for practitioners as well as beginners in relevant research fields.

Graphical abstract: Fluorogenic probes for disease-relevant enzymes

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Publication details

The article was received on 23 Aug 2018 and first published on 06 Dec 2018


Article type: Review Article
DOI: 10.1039/C7CS00907K
Citation: Chem. Soc. Rev., 2019, Advance Article
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    Fluorogenic probes for disease-relevant enzymes

    J. Zhang, X. Chai, X. He, H. Kim, J. Yoon and H. Tian, Chem. Soc. Rev., 2019, Advance Article , DOI: 10.1039/C7CS00907K

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