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Issue 88, 2019
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Pyrococcus furiosus Argonaute-mediated nucleic acid detection

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Abstract

We demonstrate that short single-stranded DNA generated by Pyrococcus furiosus Argonaute (PfAgo) can initiate a second round of cleavage. Based on this principle, we established a molecular diagnostic method, termed PfAgo-mediated Nucleic acid Detection (PAND). This method could detect DNA at attomolar sensitivities, distinguish single-nucleotide mutants and accomplish multiplexed detection.

Graphical abstract: Pyrococcus furiosus Argonaute-mediated nucleic acid detection

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Article information


Submitted
18 Sep 2019
Accepted
01 Oct 2019
First published
02 Oct 2019

Chem. Commun., 2019,55, 13219-13222
Article type
Communication

Pyrococcus furiosus Argonaute-mediated nucleic acid detection

R. He, L. Wang, F. Wang, W. Li, Y. Liu, A. Li, Y. Wang, W. Mao, C. Zhai and L. Ma, Chem. Commun., 2019, 55, 13219
DOI: 10.1039/C9CC07339F

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