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Selective and sensitive fluorescence “turn-on” detection of 4-thiouridine in nucleic acids via oxidative amination

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Abstract

A fluorescence “turn-on” method for digestion-free analysis of 4-thiouridine (s4U) in nucleic acids was developed in this work based on the oxidative amination of s4U by fluoresceinamine (FAM-NH2) and periodate (IO4). It was 125-fold more sensitive for s4U detection than the traditional UV330 absorption method, and showed excellent selectivity to s4U over 2-thiouridine (s2U) analogues and biological thiols.

Graphical abstract: Selective and sensitive fluorescence “turn-on” detection of 4-thiouridine in nucleic acids via oxidative amination

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Publication details

The article was received on 14 Aug 2019, accepted on 07 Oct 2019 and first published on 09 Oct 2019


Article type: Communication
DOI: 10.1039/C9CC06312A
Chem. Commun., 2019, Advance Article

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    Selective and sensitive fluorescence “turn-on” detection of 4-thiouridine in nucleic acids via oxidative amination

    J. Wang, J. Shang, Z. Qin, A. Tong and Y. Xiang, Chem. Commun., 2019, Advance Article , DOI: 10.1039/C9CC06312A

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