A ribonuclease-dependent cleavable beacon primer triggering DNA amplification for single nucleotide mutation detection with ultrahigh sensitivity and selectivity

Xiong Ding; Kun Yin; Ju Chen; Kepeng Wang; Changchun Liu

doi:10.1039/C9CC06296C

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A ribonuclease-dependent cleavable beacon primer triggering DNA amplification for single nucleotide mutation detection with ultrahigh sensitivity and selectivity†

Xiong Ding,^a Kun Yin,^a Ju Chen,^b Kepeng Wang^b and Changchun Liu

*^a

Author affiliations

* Corresponding authors

^a Department of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
E-mail: chaliu@uchc.edu

^b Department of Immunology, School of Medicine, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, USA

Abstract

We described a ribonuclease-dependent cleavable beacon primer, an energy-transfer-tagged oligonucleotide inserted with a ribonucleotide, which can be cleaved by ribonuclease to generate enhanced fluorescence signals and initiate DNA amplification for single nucleotide mutation detection with ultrahigh sensitivity and selectivity.

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Article information

DOI: https://doi.org/10.1039/C9CC06296C
Article type: Communication
Submitted: 13 Aug 2019
Accepted: 25 Sep 2019
First published: 25 Sep 2019

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Chem. Commun., 2019,55, 12623-12626

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A ribonuclease-dependent cleavable beacon primer triggering DNA amplification for single nucleotide mutation detection with ultrahigh sensitivity and selectivity

X. Ding, K. Yin, J. Chen, K. Wang and C. Liu, Chem. Commun., 2019, 55, 12623 DOI: 10.1039/C9CC06296C

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Chemical Communications

A ribonuclease-dependent cleavable beacon primer triggering DNA amplification for single nucleotide mutation detection with ultrahigh sensitivity and selectivity†

Abstract

Supplementary files

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A ribonuclease-dependent cleavable beacon primer triggering DNA amplification for single nucleotide mutation detection with ultrahigh sensitivity and selectivity

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